In the past couple of weeks, we have been running water column samples as well as learning how to use integration software in order to determine the nitrate+ nitrite concentration in the samples. I’ve gained an understanding of how to calculate the concentration in samples by calibrating samples against a series of standards. We inject a series of known nitrate concentrations, then use this data to create an x-y plot (area of the peak on the x-axis and concentration on the y-axis). We then add a trend line to the plot and use the equation for the slope of the line f(x)=mx+b, where concentration is a function of the area under the peaks we find using the integration software.
Additionally, we’ve begun to prep samples for total dissolved nitrogen (TDN which is the sum of nitrate, nitrite, ammonium and dissolved organic nitrogen) concentration analysis using persulfate oxidation of TDN to nitrate (Knapp et al.,2005). It’s difficult for me to elaborate more on these techniques because I’m still reading the primary literature, never the less, this is the direction our lab is headed for the next couple of weeks.
I hope that everyone’s research is going well!!!!!
Florida State University