Friday, May 23, 2014

Herbert Johnson's Internship, Summer 2014 - Part 1

Hi everyone!

My name is Herbert Johnson and I am participating in the Deep-C program at Florida State University under the supervision of Dr. Angela Knapp. This summer we plan to investigate nutrient cycling in the Gulf of Mexico. We are going to measure these nutrients in water samples to characterize the nitrogen cycle in the Gulf of Mexico. We will measure the nitrogen concentration in samples using a chemiluminesence analyzer and using other data sets and comparing nutrient distributions with Redfield stoichiometry.

The Chemiluminescent detector.
Making a concentration series of nitrate.
So far, we have been learning how to set up laboratory equipment and testing/trouble shooting the instruments and software we will be working with this summer. I’ve learned how to make a vanadium III reagent which will be used as a reducing agent for our nitrate samples as well as stock solutions of nitrate at different concentrations which will be used as standards once we run samples. I’ve also been learning more about the marine nitrogen cycle and its importance by reading the primary literature. Additionally, I’ve explored existing data nutrient concentration sets including World Ocean Atlas and those stored at the Biological and Chemical Oceanography Data Management Office (BCO-DMO).

I’m really excited about learning more over the summer!!

Posted By:
Herbert Johnson
Florida State University

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