Monday, July 8, 2013

Summer 2013 Internship: Joint Post #6 from Kala Marks and Curtis Okolovitch

This week, Kala and I have been extracting the petroleum hydrocarbons from our cultures of Acientobacter sp. and Alcanivorax sp. with 5% Macondo Oil (v/v). However, we have hit a few roadblocks in our extraction. But after throwing around ideas with other members of the lab and trying new procedures, we may finally have a working method for extracting hydrocarbons from our cultures!

Kala removing the organic solvent layer containing the hydrocarbons from one of our cultures.
In addition, our cultures of Acinetobacter sp. and COREXIT are showing some growth! In order to quantify this growth, we have been using microscopy to count these cells and estimate the total amount in the culture. Though counting is not the most enjoyable task in the lab, being able to see our strain of Acinetobacter sp. under a microscope for the first time was truly amazing.

Two of our cultures innoculated with Acinetobacter sp. The one on the left contains no COREXIT and thus has little bacterial growth. The flask on the right contains COREXIT and a ring of biomass indicating growth.
This will be the last joint post from Kala and I, as Kala is leaving next week for Israel. However, I will still be here for the next couple of weeks to keep you all updated on what’s happening in the Kostka Lab. We hoped that you enjoyed reading posts from the Deep-C Duo!

The Deep-C Duo
(Kala & Curtis, Georgia Tech)

No comments:

Post a Comment