We also finished up DNA extractions from four different sample sets and sent them off for sequencing! Curtis kind of had a crash course in microbiology this week. He did DNA extractions and PCR for the first time, and he also learned how to take growth measurements from liquid cultures. So far so good!
Until next time,
Curtis & Kala (a.k.a. “The Deep-C Duo”)
Photos (from top): 1. This is the spectrophotometer we use to measure the growth of our cultures. 2. This is our uninoculated control. There is no growth and the oil still forms a smooth sheen on the surface of the water. 3. The strain on the left is Alcanivorax and the strain on the right is Acinetobacter. Both are well known oil-degrading bacteria and grow very quickly on oil. There are differences in how they degrade oil. As seen in the picture, Acinetobacter forms more clumps around the oil whereas Alcanivorax doesn’t.