Monday, May 13, 2013

Chelsea McCurry's Internship, Summer 2013 - Part 2

Last week research progressed as expected. I am encountering issues with a few sediment samples. I am not sure if the target species is absent or if I am encountering polymerase chain reaction (PCR) inhibitions. I look forward to getting data back in the coming week.

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Chelsea McCurry

2 comments:

  1. Ms. McCurry,
    Great hyperlink! I had no idea that you could denature and synthesize DNA up to 30 or even 40 times! How many times on average do you go through that procedure with your samples?

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    Replies
    1. Well, normally we run 30-35 cycles of PCR, as for additional cycles may create biases (i.e. Suzuki et al.) with respect to the relative amount or ratio of common and rare targets present in the sample.

      The procedure does not take very long.

      Best,
      Chelsea

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